Isolation and Identification of Microorganisms


The customers are obliged to agree with the terms and conditions and should follow instructions for sending samples for isolation and identification at Thailand Bioresource Research Center (TBRC).


Download terms and conditions (TH / EN)

Download instructions for sending samples (TH / EN)

1.
  1. The customer is required to submit a service form together with the sample. For both services, the customer must complete and make payment for the isolation service before submitting a request for identification service.

2. Acceptable samples
2.1.
  1. TBRC only accepts samples from which bacteria, yeasts or filamentous fungi need to be isolated according to the protocols and conditions stated in the service form. TBRC does not accept sample of patient or diseased animal origins. Fresh mushrooms in the genera Agaricus and Boletus or samples that easily degenerate are not accepted as contamination with undesirable microorganisms usually occurs. TBRC does not accept samples from overseas.
2.2.
  1. TBRC accepts pure isolates of most groups of bacteria, yeasts and filamentous fungi for molecular identification (DNA sequence analysis) and only accepts pure isolate of bacteria and yeasts for MALDI-TOF identification. TBRC does not accept isolates of patient or diseased animal origins. TBRC does not accept isolates of diseased plant origins from overseas. If strains received from customers appeared contaminated after observation by unaided eyes, the customer will be asked to resend the strains or strain purification service can be requested at an extra charge.

3. Service and report schedule
3.1.
  1. For isolation service, please contact TBRC to arrange the shipment of samples. Isolation result will be sent to the customer within 15 days for bacteria and yeasts and 30 days for filamentous fungi and actinomycetes.
3.2.
  1. Pure isolates for identification should reach TBRC during the 1st–7th day of the month. Isolates that are not ready to be analyzed after receipt and required further processing that delay the start of the identification service will be processed together with isolates received a month later.
3.2.1.
  1. Molecular identification results of pure isolates of bacteria and yeasts received on the 1st–7th day of the month will be finished within the 7th day of the following month while pure isolates of filamentous fungi will be finished within the 7th day of 2 months later.
3.2.2.
  1. MALDI-TOF identification results of pure isolates of bacteria and yeasts received on the 1st–7th day of the month will be finished within 15th day of the month.
3.3.
  1. For identification service, the customer could request a priority service at an extra charge. Priority service is only available for bacteria (except actinomycetes) and yeasts.
3.3.1.
  1. Molecular identification report will be sent to the customer approximately 14 days after pure isolates reach TBRC.
3.3.2.
  1. MALDI-TOF identification report will be sent to the customer approximately 1 day after TBRC obtains pure and fully active isolates (1-day old for fast growing strains and 2-3-day old for slow growing strains).
3.4.
  1. Report will be sent to the customer after TBRC receives a full payment. Please inform TBRC if purchase order (PO) is required by the customer’s organization.

4. Handling of samples at TBRC
4.1.
  1. After observation by unaided eyes, acceptable samples will be processed according to customer’s requests. Rejected samples will be discarded in 7 working days after TBRC informs the customer.
4.2.
  1. Original samples will be discarded after finishing wet laboratory processes.
4.3.
  1. After isolation process, TBRC will inform the customer to submit a request for identification service and/or collect isolated microorganisms. TBRC will discard the isolated microorganisms if TBRC receive no response from the customer in 7 working days.

5. Handling of analyzed results
  1. TBRC stores analyzed results 1 year from the service request date. In case of loss of report, the customer can request a copy of the report without service fee.

6. Disclaimer
6.1.
  1. TBRC shall reserve the right to decide whether to accept or reject samples.
6.2.
  1. The results obtained from the service are for the test specimens and specified conditions only and cannot be used to certify goods not tested. The National Center for Genetic Engineering and Biotechnology (BIOTEC) will not take any responsibility for any consequence or damage which may result from information obtained from the service. Please note that BIOTEC is not a certification body. Use of the center name or symbol (logo) in any case without written permission from BIOTEC is prohibited.
6.3.
  1. Some microorganisms may not be identified to species or genus level using methods offered and/or database currently available. Molecular identification results will be reported at the higher taxon affiliation as accurately as possible based on result obtained and sequence database available at the date of report. MALDI-TOF results will be reported as unidentified species.
6.4.
  1. The customer shall provide payment as indicated in the invoice. The bank transfer fee shall be paid by the customer.
6.5.
  1. The customer is responsible for any charges occurring from shipment of samples to TBRC.
6.6.
  1. In the case that identification result cannot be reported due to sample contamination (that was not detected by visual observation by unaided eyes and only found out after analysis), full service payment will be charged by TBRC.
6.7.
  1. TBRC shall reserve the right to terminate the service and give no refund if the customer does not response to notification from TBRC accordingly.
1. Preparation of documents

The service form and proof of customer identity must be submitted to TBRC together with the sample.

    1.1. For government organizations, state enterprises or other state agencies, the form must be signed by an authorized person and submitted with a certified copy of an official identification card.
    1.2. For government officers, state enterprise employees or government employees, please attach a certified copy of an official identification card.
    1.3. For students, including post doctoral associates, the form must be signed by his/her supervisor and submitted with a certified copy of the supervisor’s official identification card.
    1.4. For individuals, please attach a certified copy of an identification card.
    1.5. For corporations, the form must be signed by an authorized person and stamped (if a company stamp is available) and submitted together with a certified copy of an authorized person’s identification card.

2. Preparation of samples

2.1. Samples for isolation of bacteria, yeasts or filamentous fungi

2.1.1. Packaged products

  1. Send 1 small package/sample (no less than 10 g or 10 ml but not exceeding 1 kg or 1 L). The product name and manufacture date/product lot should be clearly labeled on the package.

2.1.2. Liquid samples

  1. Provide no less than 10 ml per type of microorganism to be isolated/isolation method. Samples must be properly packed in leak- and breakage-proof containers.

2.1.3. Dried or viscous samples

  1. Samples should be soluble in water or easily grounded/cut into small pieces. Provide no less than 10 g per type of microorganism to be isolated/isolation method. Samples must be properly packed in leak- and breakage-proof containers.

2.2. Pure isolates for identification

2.2.1.
  1. Grow microorganisms on suitable dry-surface agar plates to reduce risk of contamination during shipment.
2.2.2.
  1. For bacteria and yeasts, cross streak on agar plates to obtain single colonies (for observation of purity before analysis) and send to TBRC within 3 days after inoculation. Send 1 plate per strain of microorganism.
2.2.3.
  1. For sporulating filamentous fungi, inoculate on sporulation media and send to TBRC within 5 days after inoculation. For non-sporulating filamentous fungi, grow on suitable growth media until colony diameter is greater than 3 cm. Send 1 plate per strain of microorganism.
2.2.4.
  1. For MALDI-TOF identification, the customer send 1 clean agar plate per strain of microorganism as subculture may be required in order to obtain young and fully active culture for analysis.

3. Shipment of samples to TBRC
  1. TBRC performs identification service in batches. Samples should reach TBRC during the 1st–7th day of the month. Samples received afterwards and those that are not ready to be analyzed after receipt and require further processing that delay the start of the identification service will be processed together with samples received a month later.

3.1. Drop-off at TBRC

Please contact TBRC in advance (Tel: +66 (0)2 117 8001, email: tbrcservice@biotec.or.th). Samples should be brought to TBRC office located at Innovation Cluster 2 Tower B, 8th Floor, Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani.

3.2. By post

3.2.1
  1. Put sample containers or agar plates in clean plastic bags (1 sample/bag). Containers of liquid samples should be put in at least 2 layers of plastic bags. Seal plastic bags properly. Wrap sample to prevent breakage and place in a cardboard box for shipment (do not pack in an envelope or a paper bag).
3.2.2.
  1. Send sample, Isolation and Identification Service Form and proof of customer identity by express mail service (EMS) to the address indicated below. The customer should take shipment times into consideration when preparing and shiping samples, as shipment may take up to 3 days from the Bangkok metropolitan region and 7 days from other areas of Thailand.


To

Thailand Bioresource Research Center (TBRC)
Attn: Microbial Isolation and Identification Service
National Center for Genetic Engineering and Biotechnology
Innovation Cluster 2 (Tower B, 8th Floor)
143 Thailand Science Park, Phahonyothin Road
Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand

Topic

Detail

Contact person

Email

Tel.

General information

Sample submission, service process, service fee, quotation, invoice, report

Kusuma Srisuk

kusuma.sri@biotec.or.th

+66 (0)2 117 8001

Technical information

Actinomycetes

Chanwit Suriyachadkun

chanwit@biotec.or.th

+66 (0)2 564 6700 ext. 3336

Bacteria

Pattaraporn Rattanawaree

pattaraporn@biotec.or.th

+66 (0)2 117 8018

Filamentous fungi

Veera Sri-Indrasutdhi

veera@biotec.or.th

+66 (0)2 564 6700 ext. 3202

Yeasts

Sasitorn Jindamorakot

sasitorn@biotec.or.th

+66 (0)2 564 6700 ext. 3333

MALDI-TOF identification

Piyanun Harnpicharnchai

Piyanun.Har@biotec.or.th

+ 66 (0)2 117 8025

Method Microorganism Details Fee (Excluding VAT)
Organizations in Thailand Overseas
Isolation Bacteria and yeasts Swab test/Serial dilution 500 Baht/sample Not available
Total plate count 500 Baht/sample Not available
Filamentous fungi Direct isolation 1,000 Baht/isolate Not available
Bacteria, yeasts and filamentous fungi Strain purification 300 Baht/isolate Not available
Preparation of isolated strain to send to customer Bacteria, yeasts and filamentous fungi Fresh culture 150 Baht/isolate/1 tube Not available
Liquid-dried ampoule 1,500 Baht/isolate/5 tubes
See Culture Preservation Service
Not available
Molecular identification of pure isolate Bacteria Single strand 16S rDNA sequence analysis (~1,000 bp) 2,500 Baht/isolate 5,000 Baht/isolate
Double strand 16S rDNA sequence analysis (~1,300 bp) 4,000 Baht/isolate 8,000 Baht/isolate
Yeasts D1/D2 domain of 26S rDNA sequence analysis (500-600 bp) 2,500 Baht/isolate 5,000 Baht/isolate
Filamentous fungi Sequence analysis of the first selected gene 3,500 Baht/isolate 7,000 Baht/isolate
Additional gene 1,500 Baht/gene 3,000 Baht/gene
MALDI-TOF identification of pure isolate Bacteria and yeasts 1,400 Baht/isolate 2,800 Baht/isolate
Priority service (Molecular identification) Bacteria and yeasts Report result approximately 14 days after receiving pure isolate 2,500 Baht/order
(Maximum 10 isolates/order)
Not available
Priority service (MALDI-TOF identification) Bacteria and yeasts Report result approximately 1 day after receiving pure isolate 2,500 Baht/order
(Maximum 50 isolates/order)
Not available
Strain purification (if isolate received is contaminated) Bacteria, yeasts and filamentous fungi 300 Baht/isolate 600 Baht/isolate
Bank transfer   Paid by the customer Paid by the customer
  • Customer fills online service form, prints out, signs and sends service form together with sample to TBRC
  • TBRC inspects sample upon receipt and informs service fee
  • Customer makes a payment
  • ​TBRC isolates/identifies sample
  • ​TBRC sends report/isolated microorganism to customer